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The data below were collected for an enzyme-catalyzed reaction. determine the vmax and km for this reaction

Ross Pratt

in Chemistry

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1 answer

Zach Chandler on August 1, 2018

The parameters vmax and km were determined using models known in the enzymatic reactions. A linear model in the enzymatic reactions is the Lineweaver-Burke model. Its form is written as1/v = (Km/vmax)(1/S) + 1/vmax,whereKm/vmax is the slope1/vmax is the y-interceptIn order to determine the parameters of the plot (1/v) vs (1/S) of data, where v is the velocity of reaction and S is the substrate concentration. Then, find the y-intercept is the point at which the line crosses the y axis. The reciprocal of the y-intercept is equal to vmax. Per Km, find the slope through linear regression. Then, multiple vmax the slope to determine Km.

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